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KMID : 1102020170470030160
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Applied Microscopy
2017 Volume.47 No. 3 p.160 ~ p.164
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Recent Advances in Electron Crystallography
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Chung Jeong-Min
Lee Sang-Min
Jung Hyun-Suk
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Abstract
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Electron crystallography has been used as the one of powerful tool for studying the structure of biological macromolecules at high resolution which is sufficient to provide details of intramolecular and intermolecular interactions at near-atomic level. Previously it commonly uses two-dimensional crystals that are periodic arrangement of biological molecules, however recent studies reported a novel technical approach to electron crystallography of three-dimensional crystals, called micro electron-diffraction (MicroED) which involves placing the irregular and small sized protein crystals in a transmission electron microscope to determine the atomic structure. In here, we review the advances in electron crystallography techniques with several recent studies. Furthermore, we discuss the future direction of this structural approach.
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KEYWORD
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Electron crystallography, Protein structure, Transmission electron microscopy, Micro-electron diffraction, Structural biology
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